Publication:
The Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodelling

dc.contributor.authorSimmons, Cameron
dc.date.accessioned2018-09-14T11:15:12Z
dc.date.available2017-11-22T23:25:42Z
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2017-10-12en_US
dc.date.available2018-09-14T11:15:12Z
dc.date.issued2017-11-07en_US
dc.description.abstractFlaviviruses are enveloped, positive-sensed single-stranded RNA viruses that remodel host membranes, incorporating both viral and host factors facilitating viral replication. In this study, we identified a key role for the membrane-bending host protein Reticulon 3.1 (RTN3.1A) during the replication cycle of three flaviviruses: West Nile virus (WNV), Dengue virus (DENV), and Zika virus (ZIKV). We observed that, during infection, RTN3.1A is redistributed and recruited to the viral replication complex, a recruitment facilitated via the WNV NS4A protein, however, not DENV or ZIKV NS4A. Critically, small interfering RNA (siRNA)-mediated knockdown of RTN3.1A expression attenuated WNV, DENV, and ZIKV replication and severely affected the stability and abundance of the NS4A protein, coinciding with a significant alternation and reduction of viral membrane structures in the endoplasmic reticulum. These observations identified a crucial role of RTN3.1A for the viral remodelling of host membranes during efficient flavivirus replication and the stabilization of viral proteins within the endoplasmic reticulum.
dc.identifier.urihttps://demo7.dspace.org/handle/10673/236
dc.languageEnglishen_US
dc.titleThe Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodellingen_US
dc.typeJournal Article
dspace.entity.typePublication
relation.isAuthorOfPublicationb1b2c768-bda1-448a-a073-fc541e8b24d9
relation.isAuthorOfPublication.latestForDiscoveryb1b2c768-bda1-448a-a073-fc541e8b24d9
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