Publication: MHC class I-restricted cytotoxic lymphocyte responses induced by enterotoxin-based mucosal adjuvants
MHC class I-restricted cytotoxic lymphocyte responses induced by enterotoxin-based mucosal adjuvants
| dc.contributor.author | Simmons, Cameron | |
| dc.date.accessioned | 2018-09-14T11:15:06Z | |
| dc.date.available | 2017-07-05T02:44:24Z | |
| dc.date.available | 2018-09-14T11:15:06Z | |
| dc.date.issued | 1999-12-15 | en_US |
| dc.description.abstract | The ability of enterotoxin-based mucosal adjuvants to induce CD8+ MHC class I-restricted CTL responses to a codelivered bystander Ag was examined. Escherichia coli heat-labile toxin (LT), or derivatives of LT carrying mutations in the A subunit (LTR72, LTK63), were tested in parallel with cholera toxin (CT) or a fusion protein consisting of the A1 subunit of CT fused to the Ig binding domain of Staphylococcus aureus protein A (called CTA1-DD). Intranasal (i.n.) immunization of C57BL/6 mice with CT, CTA1-DD, LT, LTR72, LTK63, but not rLT-B, elicited MHC class I-restricted CD8+ T cell responses to coadministered OVA or the OVA CTL peptide SIINFEKL (OVA257-264). CT, LT, and LTR72 also induced CTL responses to OVA after s.c. or oral coimmunization whereas LTK63 only activated responses after s.c. coimmunization. rLT-B was unable to adjuvant CTL responses to OVA or OVA257-264 administered by any route. Mice treated with an anti-CD4 mAb to deplete CD4+ T cells mounted significant OVA-specific CTL responses after i.n. coadministration of LT with OVA or OVA257-264. Both 51Cr release assays and IFN-gamma enzyme-linked immunospot assays indicated that IFN-gamma-/- and IL-12 p40-/- gene knockout mice developed CTL responses equivalent to those detected in normal C57BL/6 mice. The results highlight the versatility of toxin-based adjuvants and suggest that LT potentiates CTL responses independently of IL-12 and IFN-gamma and probably by a mechanism unrelated to cross-priming. | |
| dc.identifier.uri | https://demo7.dspace.org/handle/10673/201 | |
| dc.language | English | en_US |
| dc.title | MHC class I-restricted cytotoxic lymphocyte responses induced by enterotoxin-based mucosal adjuvants | en_US |
| dc.type | Journal Article | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | b1b2c768-bda1-448a-a073-fc541e8b24d9 | |
| relation.isAuthorOfPublication.latestForDiscovery | b1b2c768-bda1-448a-a073-fc541e8b24d9 |