Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds

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Date
2000
Authors
Roy, Ipsita
Sardar, Meryam
Gupta, Munishwar N
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Abstract
Two polysaccharides, alginate and chitosan, showed unusual affinity and bound a-amylase (from various sources) and Aspergillus niger cellulase, respectively. The beads prepared from these polymers were successfully used for the purification of the respective enzymes by fluidized bed affinity chromatography. a-amylase from wheat germ could be purified by 58-fold with about 90% recovery of activity.Aspergillus niger cellulase, on the other hand, was purified by 30-fold with 80% recovery of enzyme activity. Both purified preparations show single band on SDS-PAGE.
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Fluidized bed affinity chromatography, Polysaccharides, Bioseparation, Carbohydrases, a-amylase, cellulase
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